EFFECT OF ADDING GROWTH INHIBITORS ON THE QUALITATIVE CHARACTERISTICS OF MICROTUBERS OF FOUR POTATO VARIETIES IN VITRO

Abstract

The experiment was carried out in the plant tissue culture laboratory of the Nakheel Garden Tissue Agriculture Co., Ltd., Baghdad, for the period from March 2022 to November 2022. Its purpose was to study the effect of adding different concentrations of paclobutrazol and coumarin on the qualitative characteristics of fine tubers and four varieties of potatoes, as the experiment included four Treatments of all paclobutrazol and coumarin, which are as follows: four levels of paclobutrazol at concentrations (0, 3, 6, and 9 mg L), respectively, and four levels of coumarin at concentrations (0, 10, 15, and 20 mg L), respectively, and four varieties of potatoes, as follows (Arizona and Burren). (Hermiz and Spunta) and analyzed the evidence from the first experiment as a factorial experiment using a completely randomized design (CRD). Adding paclobutrazol at a concentration of 3 mg. L to the MS nutrient medium led to a significant increase in the percentage of carbohydrates. Estimation of the effectiveness of the peroxidase enzyme. Percentage of reducing sugars. Percentage of protein and nitrogen in the fine tubers. Adding coumarin at a concentration of 10 mg per liter to the MS nutrient medium led to a significant increase in the percentage of carbohydrates. Estimation of the effectiveness. Peroxidase enzyme, percentage of reducing sugars, percentage of protein and nitrogen in fine tubers. The interaction between paclobutrazol and coumarin, paclobutrazol and varieties, coumarin and varieties, and the triple interaction led to a significant increase in most of the traits studied.