EFFECT OF ADDITION OF PACLOBUTEROL AND COUMARIN ON THE PRODUCTION OF MICROTUBERS OF FOUR POTATO CULTIVARS IN VITRO
Keywords:
potato, vitro, micro-tubersAbstract
A field experiment was carried out in the plant tissue culture laboratory of the Nakheel Paradise Company for Tissue Agriculture Ltd., Baghdad, for the period from March 2022 to November 2022. Its purpose was to study the effect of adding different concentrations of paclobutrazol and coumarin on the production of micro tubers and four varieties of potatoes, as the experiment included four treatments of All paclobutrazol and coumarin, which are as follows: four levels of paclobutrazol in concentrations (0, 3, 6, and 9 mg/L, respectively), and four levels of coumarin in concentrations (0, 10, 15, and 20 mg/L), respectively, and four varieties of potatoes, which are as follows: Arizona, Burren, and Spuntas Hermiz. The data from the first experiment was analyzed as a factorial experiment using a completely randomized design (CRD). Adding paclobutrazol at a concentration of 3 mg. Addition of paclobutrazol at a concentration of 9 mg. liter to the nutrient medium led to an increase in the proline content in the tubers, and adding coumarin at a concentration of 10 mg liter 1 to the MS nutrient medium led to a significant increase in the weight of the micro-tuber, the number of micro-tubers, and the diameter of the micro-tubers, and adding coumarin at a concentration of 20 mg liter to the nutrient medium led To increase the proline content in tubers, the interaction between paclobutrazol and coumarin, paclobutrazol and varieties, coumarin and varieties, and the triple interaction led to a significant increase in most of the traits studied
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