THE EFFECT OF COMBINATIONS OF GROWTH REGULATORS ON THE ELONGATION OF THE TISSUE STEMS OF THE MEDJOOL DATE PALM AND THE USE OF RHABDITE TECHNOLOGY TO DETECT GENETIC CHANGES IN THE TISSUE DEFORMED LEAVES

Abstract

The effect of combinations of growth regulators on the elongation of the tissue branches of the Medjool date palm, where the T5 treatment (1 mg L-1 of each of Kin, 2iP, BA, IAA) outperformed most of the experimental characteristics by giving the highest values in terms of the average number of new leaves (2.66 leaves per plant part-1 ). ) and the percentage of shoots rooting (80%) and the average of; number of roots (6 roots per plant part - 1), shoot length (2.66 cm), and increase in stem length (4.33 cm). Genetic variations (one of the major production defects in date palm tissue farms) resulting from the tissues of the unknown variety of date palms produced by the indirect organogenesis method were studied to show the ratio of genetic differences between the distorted and normal leaves in the tissue to indicate their exclusion from the micropropagation protocol using the rhabd technology to detect mutations. Using seven random primers after amplification of the total DNA, three primers revealed polymorphisms and gave results in detecting heterogeneity, as the study showed a genetic difference of 10% between the two distorted tissues and the normal plant.