EFFECTS OF THE DIFFERENT (2IP) CONCENTRATIONS ON CITRUS LEMON L. SHOOT MULTIPLICATION AND IBA ON ROOTING IN VITRO

Abstract

The researchers wanted to see how varying concentrations of (2iP) affected the growth and multiplication of Citrus shoots in vitro. This study was conducted in the Horticulture and Landscape Department, College of Agriculture, University of Kirkuk 2020's plant tissue culture laboratory. Citrus explants were grown on MS media supplemented with different quantities of (2iP) 0, 1.0, 1.5, 2.0, and 2.5 mg L-1, according to the design (CRD). The results suggest that (2iP) had an effect on in vitro growth and multiplying shoots, with the largest number of shoots being 1.8 and 2.0 shoot.explant-1 at 2.50 mg. After 4 and 8 weeks of culture, L-1 2iP was obtained. In addition, the concentration exceeded 2.5 mg.L-1 in the rate of length (1.2, 1.6) cm and number (1.2, 1.6)cm after 4 and 8 weeks of cultivation, cm and number of leaves (3.3,4.3) leaf.explant-1 were measured, compared to 0 concentration, which had the lowest rate