ISOLATION AND IDENTIFICATION OF HYDATID CYST FLUIDASSOCIATED BACTERIA ISOLATED FROM BUFFALO IN THE SOUTHERN OF LRAQ

Authors

  • Ghufran S.Jabber. AL-Diwan Department of Biology , Collegy of Education in Qurina, University of Basrah, Basrah, Iraq
  • Adnan. B.G. AL-Hawash Department of Biology, College of Education for Pure Sciences, University of Basrah, Basrah. Iraq
  • Sarmad .A.M.AL-Asadi Department of Biology, College of Education for Pure Sciences, University of Basrah, Basrah. Iraq

Keywords:

Echinococcus granulosus, hydatid cyst fluid, associated bacteria

Abstract

Echinococcus granulosus is known as the causative agent of hydatid disease. It belongs to the family Platyhelminthes within the family Taeniidae. Infected hydatid cysts are isolated from intermediate hosts. Fifty samples of liver and lung of buffaloes infected with hydatid cysts were collected during the period from February 2022 to March 2022 from southern Iraq. The aim of the study was to know the types of bacteria associated with hydatid cysts and isolated from the livers and lungs of buffaloes for the purpose of verifying this. The diagnosis was made by several tests, including phenotypic, biochemical and microscopic diagnosis, and then the antibiotic was tested To make sure of the tests, vitek test. The hydatid cyst fluid isolated from the lungs was 100%, and its percentage was in the liver, where it constituted 96%. The results of the biochemical diagnosis were E.coli, Shigella spp, Proteus spp, Providencia, and Klebsiella. For confirmation, two isolates isolated from the lungs were diagnosed using Vitek. It belonged to Acinobacter lwoffii and Providencia rettgerii, where the results were identical to the genus Providencia and contrary to the genus Acinobacter in the biochemical diagnosis, with the exception of the genus Providencia spp. As for the liver, four isolates were diagnosed and the results showed that they belong to Mollerea wisconsensiss, Pseudomonas fluorescens, Shingomonas paucimobilis and E. coli. The result was that there was no match between them and the biochemical diagnosis. Also, the result was that there was no match between them and the biochemical identifaction. As for the antibiotic sensitivity test, the most sensitive isolates to antibiotics were Gentamicin, Ceftazidim, Cefepim Imipenem, Ciprofloxacin, Amikain, Trimethoprim / sulfamethoxazole, Piperacillin / Tazobactam, Ticarcillin / Clavulanic, Ticarcillin / clavulanic acid, Ticarcillin, Imipenem

References

Abdulhameed,M. F., Robertson, I.D., Al-Azizz, S. A., & Habib,I. (2019). Neglected Zoonoses and the Missing Opportunities for One Health Education: The Case of Cystic Echinococcosis among Surgically Operated Patients in Basrah, Southern Iraq.Diseases, 7(1),4.

Alhasany, A. R., Noaema, A. H., & Alhmadi, H. B. (2019, July). The role of spraying copper and zinc on the growth and yield of Vicia faba L. In IOP Conference Series: Materials Science and Engineering (Vol. 571, No. 1, p. 012048)

Darweesh AO, Shareef AA, Alriyahee F.A.A.(2021). Isolation and Identification of Cellulytic Ruminant Pseudomonas Aeruginosa by classical and Molecular Methods in Basrah Province. Ann Rom Soc Cell Biol.4672-80.

Dew HR (1928). Hydatid disease. Its Pathology ,diagnosis and treatment The Australasian Medical Publisling Company,Limited Sydney.

Eckert J,Deplaze P(2004). Biological ,epidemiological, and clinical aspects of Echinococcosis, azoonosis of in creasing concern,Clin Microbiol Rev 17:107-135.

Fallah ,M, Kavand A, Yousefi Mashouf R(2014). Infected hydatid Cysts bacteria in Slaughtered linestock and their effects on protoscoleces degeneration. JAUNDISHAPUR J Microbiol 7:e10135.

Jahed Khaniki G.R.,Kia E.B, Raei M.(2013).Liver condemnation and economiclosses due to parasitic in Slaughtered animal in Iraq. J.Parasit Dis 37:240-244.

Hammad, S.J. (2017). Detection of Echinococcus granulosus strains using morphological parameters and molecular methods. Ph.D.Thesis, College of Science, Univeersity of Tikrit. Iraq.pp:48-150.

Markey,B.; Leonard, F.; A rchambanlt , M,; Cullinane, A. and Maguire, D.(2013). Clinical veterinary microbiology E- Book-Elsevier Health sciences.

Levinson, W .(2016). Review of Medical Microbiology and Immunology . 14 th ed. McGraw-Hill education , Inc. pp821

Max , A , Timothy, J., Barbara , H., Thomas, V. and Barbara ,J . (2011). Antimicrobial susceptibility of Aeromonas spp. Isolated from clinical and environmental sources to 26 antimicrobial agents. J. Antimicrob . Agents Chemother , 56, p. 1110-1112.

Quinn, P.J.; Markey,B. K. Carter,M.E.; Donnelly,W.J. and Leonard, F. C. (2004). Veterinary microbiology and microbial Diseases . 1 st Ed.; Blackwell Science Ltd.; PP:327.

Sadjjadi S M(2006).Present situation of echinococcosis in middle East and Arabic North Africa. Parasitol Int 55(Suppl):S197-202.

Tille ,P.M.(2017). Baily And Scotts Diagnostic Microbiology. 14 th ed. Elsevier,Inc. China. 1115pp.

Ziino G. Giuffrida, Bilei .S, Panebianco ,A. (2009). Bacterial isolates from25 hydatid cysts in sheep, cattle, and goats, Vet Rec 165:234-6.

Downloads

Published

2023-03-24

How to Cite

Ghufran S.Jabber. AL-Diwan, Adnan. B.G. AL-Hawash, & Sarmad .A.M.AL-Asadi. (2023). ISOLATION AND IDENTIFICATION OF HYDATID CYST FLUIDASSOCIATED BACTERIA ISOLATED FROM BUFFALO IN THE SOUTHERN OF LRAQ. European Scholar Journal, 4(3), 115-118. Retrieved from https://scholarzest.com/index.php/esj/article/view/3319

Issue

Vol. 4 No. 3 (2023): ESJ

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.