DETERMINATION OF IGG ANTIBODY AGAINST LEUKEMIA VIRUS IN CATTLE USING IMMUNOENZYME ASSAY

Abstract

Cattle leukemia is a chronic infectious disease that results from the poisoning of blood cells by the virus, leading to disruption of the genetic apparatus and poor cell quality. The proposed IFA method allows the detection of specific antibodies in infected animals from 6 months of age and older (calves fed oral milk and milk from infected cows can receive maternal antibodies stored for up to 6 months), so false positive results when serologically tested young calves can be obtained. It is also used in rapid screening to detect the presence of infected animals in the herd (up to 10 mixed serums are allowed in groups), and is used to confirm PTsR results when provirus DNA levels are minimal.