STUDY THE ROLE OF ADENINE SULFATE, SUCROSE AND PLANT GROWTH REGULATORS FITTED TO THE MEDIA PHYSIOLOGICALLY IN MICROPROPAGATION OF MELISSA OFFICINALIS L. PLANT IN VITRO

Abstract

Susceptibility of a number of factors were tested in the stages of propagation of the lemon balm plant Melissa officinalis under controlled conditions in the laboratory of plant tissue, Horticulture and Gardening department , Agriculture college, Karbala university, shoots culture of Melissa were received from planting the ends of the branches with a length one cm on MS medium that have been prepared with different concentrations (0.0, 0.5, 1.0, 2.0) mg l-1 of adenine sulfate and (0.0, 1.0, 2.0, 3.0) mg l-1 of benzyl adenine with fixed con. 0.5 mg l-1 of IBA, The rooting experiment included the cultivation of vegetative branches with a length of two cm obtained from the multiplication stage on a nutrient medium prepared for rooting, which includes different con. (20, 30, 40) g/l of sucrose and (0.0, 0.5, 1.0, 1.5) mg l-1 of IBA with a constant con. 0.5 mg l-1 IBA, results indicated that superiority of adenine sulfate at con. one mg l-1 in investigation the top average length of vegetative branches was 5.40 cm, while the con. two mg l-1 was superior in achieving the highest average of shoot number, fresh and dry weight of total shoot was 31.75 branches plant-1 , 4.51 and 0.95 mg consecutively, and about the influence of BA on the average of the studied traits of the vegetative group, it was superior at the con. two mg l-1 in achieving the highest average length of branches and dry weight of total vegetative was 3.61 cm. and 0.80 mg consecutively. whereas the con. of 3 mg / liter achieved the highest average branches number and the fresh weight of the vegetative total was 30.24 branches plant-1 and 4.19 mg, respectively, and on the effect of sucrose on the studied root traits characteristics, the con. 40 g l-1 exceeded in height average, roots number, wet and dry weight of rooting group reached 4.00 cm and 27.03 roots branches-1 , 2.35 and 0.57 mg respectively, as the results indicated the superiority of the IBA with a concentration of 1 mg / Liter in the mean root mean rate was 4.00 cm, 24.66 roots / branches, 2.34 and 0.51 mg, respectively.