EVOLUTION OF A METHOD FOR CALLUS INDUCTION OF STRAWBERRY (FRAGARIA ANANASSA DUCH)
Keywords:
Strawberry, Growth Regulators, Callus InductionAbstract
The aim of this study is to generate callus, while the aim of the experiment is to explore the influence of growth regulators on callus formation in young and mature strawberry leaves (Childers and Rosa Linda). The initiation of Callus from the meristematic cells of both Chandler and 'LiNa' varieties was achieved. Various concentrations of NAA and Kin on Ms media, under dark and light conditions, were used to induction callus. The results showed that the greatest number of callus formation in Chandler and Rosa Linda occurred in the medium of multiple sclerosis that had 2 mg/l NAA with 0.3 mg/l Kin in dark conditions. Additionally, our protocol employs meristems as the source of detection for the induction of callus in both types of meristems. The protocol described in this study can be valuable in creating an in vitro system for strawberries, which holds great potential for genetic transformation and crop improvement.
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