EFFECT OF BAP CONCENTRATION AND CONTENT OF FOOD ENVIRONMENT ON “IN VITRO” REGENERATION OF RIZAMAT (VITIS VINIFERA L) CULTIVAR

Abstract

This article identifies the composition of the nutrient medium and the amount of new concentrations for the development of local grape varieties of Uzbekistan in vitro. In the experiment, MS and DKW media were used, auxin NAA in the amount of 0.01 and 0.5 mg / l, and cytokinin BAP in the amount of 0.1; 0.5; 1; used at a concentration of 2 mg / l. According to the results, the best development parameters of the variety are MS 4,4 mg / l + NAA 0,01 mg / l + BAP 0,5 mg / l when applied at a concentration of 3 cm, 93.5%, root formation was 4 mm, 89,3%, buds while the length was 1.5 cm, 81.6%, the height of the implant was 2 cm, 48.5%, the most optimal values in the nutrient medium DKW 5.3 mg / l + NAA 0.01 mg / l BAP 0.1 mg / l when applied, the length of the root of the explant was 5 cm, 91.3%, colus formation was 2.5 mm, 60.1%, no budding was observed, the growth length of the implant was 7 cm, 88.7% was noted. It was found that the types of grape varieties, their morphological and physiological characteristics, as well as the parameters of development in artificial nutrient media vary. In conclusion, MS 4,4 mg / l + NAA 0,5 mg / l + BAP 0,5 mg / l nutrient medium for colus formation and bud, DKW 5,3 mg / l + NAA 0,01mg / l + BAP for growth and rooting of the explant, 1 mg / l nutrient medium is recommended