INFLUENCE OF SUBSTANCES REGULATING THE GROWTH OF LEMON IN MEYER VARIETIES FROM CITRUS PLANTS AND EARLY CULTURE PROCESSES.
Keywords:
Limon, Media, NAA, Surface SterilizationAbstract
In the Meyer variety of lemon, using L. Osbek's clonal micro-multiplication methods, the following order of use of sterilizers for sterilization of implants was found to be appropriate: 96% ethanol (5 sec) and 4% sodium hypochlorite solution (20 min). The yield under sterile conditions was 70%. A solid Murasige and Skuga (MS) nutrient medium with the addition of 1.0 mg / l benzylaminopurine and 0.5 mg / l naphthyl acetic acid was found to be optimal for in vitro propagation of the Meyer variety of lemon. Plants are planted in the soil substrate: peat, perlite, vermiculite, sand 7: 2: 3: 2 ratio. Plants adapted to the growing environment in option 1 - ½ MS +3.0 mg / l NUK plant viability was 68.2%, and in option 2 this indicator -½ MS MS = 1.0 mg / l NUK + 1.0 mg / l IUK o ' the viability of the wires was 87.5%
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