EFFECT OF PLANT HORMONES IN DIFFERENT STAGES OF IN VITRO MICROPROPAGATION OF PAULOWNIA SHAN TONG (P. TOMENTOSA & P. FORTUNEI)

Abstract

In vitro propagation conditions of Paulownia Shan Tong (P. tomentosa & P. fortunei), especially effect of 6-benzylamino purine (BAP) in multiplication media and effect of indole-3-butyric acid (IBA) in rooting media were studied. Shoot proliferation was induced by the mean of MS medium containing different concentrations of BAP (0, 0.25, 0.5 and 1 mg/L-1 ) and supplementation of the culture medium by 1.0 mg/L-1 of BAP induced the highest mean length of shoots / explants (4,38 cm) and number of nodes per shoot (5,86). For root induction, elongated shoots were transferred into MS medium supplemented with various concentrations of IBA (0, 0.25, 0.5, 1 and 2 mg/L -1 ). 98 % of shoot cuttings produced roots when they were cultured in the medium with 0.5 mg/L -1 IBA. The highest number of roots per microcutting was 5.2 and the maximum root length was 6,383 cm