EFFECT OF INOCULATION WITH PHOSPHATE-DISSOLVING BACTERIA AND FUNGI WITH HUMIC ACIDS IN THE KINETIC PARAMETERS OF THE ALKALINE PHOSPHATASE ENZYME IN CADMIUM-POLLUTED SOIL

Abstract

A laboratory experiment was carried out in the Department of Soil Sciences and Water Resources, College of Agriculture / University of Basra, to identify the role of the bacteria (Bacillus subtilis), the fungus (Aspergillus niger) isolated from agricultural areas, and humic acids extracted from cow dung in the kinetic parameters of the alkaline phosphatase enzyme in cadmium-polluted soil and compare it with soil unpolluted cadmium . The soil was treated with a cadmium sulfate solution up to the critical limit (3 mg Cd L-1 ), with the addition of humic and fulvic acid at a level of 50 L ha-1 , individually, then the soil was inoculated with bacterial B. subtilis and fungal A. niger vaccines, both individually and with a mixture of vaccines, and the activity of the enzyme alkaline phosphatase was measured under different levels of the substrate (0.010, 0.025, 0.050, 0.075 and 0.100 M) and kinetic parameters (Vmax and Km) were calculated using the Lineweaver-Burk, Hanes-Woolf and EadieHofstee equation