STUDY OF GENE EXPRESSION OF VIRULENCE FACTORS OF SOME CANDIDA FUNGAL ISOLATED FROM DIFFERENT CLINICAL CASES

Abstract

Yeasts of the genus Candida are the main causes of oral candidiasis, and Candida albicans is the most common cause. Aim of the study: This study aimed to isolate and diagnosis the yeasts that cause Oral Candida in infected children Material and methods: The study included children with oral candidiasis visiting Dhuluiya General Hospital and some private clinics in Dhuluiya during the period from December 2022 to January 2023. During this period, 100 samples were collected from children aged from 1 day to 12 years and of both sexes (males, females). . The diagnosis was made based on microscopic examination, laboratory culture of samples on saprodextrose agar medium, and the use of differentiation medium (Chrom Agar) for isolated Candida to distinguish between the types of the genus Candida spp. And diagnosis using the Vitec device. The genetic effect of silver nanoparticles prepared from the large fungus Schizophyllum Commune on the gene expression of C.albicans yeast was studied on four genes, namely CAT1, CAP1, and LIP3.Result: Most of the isolates were C.albicans, with 26 at 59.09%, followed by C.tropicalis, with 17 at 38.64%, and C.parapsilosis, with 1 at 2.27%. The genetic influence of silver nanoparticles from the fungus Schizophyllum Commune on C.albicans yeast gene expression was examined on four genes: CAT1, CAP1, and LIP3. The ALS1 gene encoding C.albicans yeast with code 91 had a stronger effect, with gene expression reaching 0.42. C.albicans with code number 92 was 0.16. Conclusion: This study concluded Isolation of three types of Candida spp. and the efficacy of silver nanoparticles made from Schizophyllum against candida albicans