BACTERIOLOGICAL AND MOLECULAR STUDY OF ANTIBIOTICS EFFECT ON GROWTH ESCHERICHIA COLI ISOLATED FROM URINARY TRACT INFECTION AND DIARRHEAL IN CALVES

Abstract

The current study included testing the effect of antibiotic effect on E. Coli bacteria isolated from cases of urinary tract infection and diarrhea in calves . 49 samples from veterinary clinics in Al-Qasim and Al-Hashimiya during the period from March to July 2022. The results showed 10 isolates of E. coli diagnosed in calves. 4 isolates (16.67%) from 24 samples UTI , while 6 isolates (24%) from 25 samples diarrhea infections in calves. The isolates were re-diagnosed according to culture tests, Gram stain and biochemical test.It was growth on the MaCconkey agar and it gave a pink color, as well as on the grom agar, it gave a green color, but on the EMBagar it gave a green metallic sheen. Antibiotic susceptibility testing was performed on isolates of Escherichia coli by kirby-bure method. The results showed that E. coli was 100% resistant to ciprofloxacin and 90% to levofloxacin, while the rest of the antibiotics were less resistant. Also, the efflux pump genes responsible for E. Coli's AcrA were detected using polymerase chain reaction (PCR) and all E. Coli isolates showed contain those genes. Therefore, this study was aimed to assess the in vitro antibiotic activity against E. coli as well as to shed spot light upon its efficacy in reducing E. coli infection in calves.