EFFECT OF ZINC, SILVER AND TITANIUM NANOPARTICLES ON THE GENES OF VIRULENCE FACTORS OF BACTERIA ISOLATED FROM DENTAL CARIES

Abstract

The sensitivity of Gram-positive and negative bacterial isolates to some types of manufactured nanomaterials was tested.Zinc, Sliver, Titanium with different concentrations, where the concentrations (100, 75, 50, 25) were used. The presence of the inhibition circuit indicated that zinc nanoparticles are a good antibacterial in varying proportions. Not all isolates appeared in response to silver and titanium nanomaterials, after which the treatment was treated with the synergistic effect. For nanomaterials on bacterial isolates, they gave better results than using them alone. The results showed the variation in the response of the isolates to the synergistic effect of the antibiotic with the nanomaterials and the difference in their sensitivity and resistance from one isolate to another. The results of the study showed using the technique ofRT-PCR differentiated response to virulence factors protein A, haemolysin, TSST to bacterial isolatesS. aureusFor the effect of zinc nanoparticles used in concentrations (2,1,0.5,0.25mg). And we got the highest inhibition at the concentration (0.5 mg).